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journal article

Two-dimensional random access multiphoton spatial frequency modulated imaging

Optics Express
Publication Date: 1/2/2020

Abstract: Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial light modulator (SLM) is used to program the excitation source, opening the door to a more versatile, random access imaging environment. In addition, an in-line, quantitative pulse compensation and measurement scheme is demonstrated using a new technique, spectral phase and amplitude retrieval and compensation (SPARC). This enables full characterization of the light exposure conditions at the focal plane of the random access imaging system, an important metric for optimizing, and reporting imaging conditions within specimens.

Alyssa M. Allende Motz
John Czerski
Daniel E. Adams
Charles Durfee
Randy Bartels
Jeff Field
Christopher L. Hoy
Jeff Squier